measurement of indicator amino acid oxidation as a method for determination of amino acid requirements in broiler breeder pullets

in this experiment two pre-laying broiler breeder pullets from ross strain at 17 weeks of age were selected, weighed and allocated in layer cages. surgery was achieved to implant a jugular vein catheter. the birds were placed individually in metabolic chamber. each bird was fed, in random order, test diets containing one of nine levels of lysine (0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.8, 1.0 and 1.5 percent of the diet). indicator oxidation was determined for primed dose (75 kbq/kgbw) and 4 hours continuous infusion of indicator (44 kbq.h-1.kgbw-1) too. 14co2 exhalation breakpoint was used to determine lysine requirement of the experimental birds. individual lysine requirement for bird 1 and 2 was 0.463 and 0.458 percent of diet, respectively. mean lysine requirements for two birds was 0.461 percent of diet.